lambda mouse 129-sv Search Results


sv mouse genomic dna library  (TaKaRa)
95
TaKaRa sv mouse genomic dna library
Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell <t>genomic</t> <t>DNA</t> hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.
Sv Mouse Genomic Dna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sv mouse genomic dna library/product/TaKaRa
Average 95 stars, based on 1 article reviews
sv mouse genomic dna library - by Bioz Stars, 2026-05
95/100 stars
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phage vector lambda embl3  (Promega)
90
Promega phage vector lambda embl3
Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell <t>genomic</t> <t>DNA</t> hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.
Phage Vector Lambda Embl3, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/phage vector lambda embl3/product/Promega
Average 90 stars, based on 1 article reviews
phage vector lambda embl3 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
random primer labeling kit  (TaKaRa)
96
TaKaRa random primer labeling kit
Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell <t>genomic</t> <t>DNA</t> hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.
Random Primer Labeling Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/random primer labeling kit/product/TaKaRa
Average 96 stars, based on 1 article reviews
random primer labeling kit - by Bioz Stars, 2026-05
96/100 stars
  Buy from Supplier
pgem 4  (Promega)
90
Promega pgem 4
Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell <t>genomic</t> <t>DNA</t> hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.
Pgem 4, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pgem 4/product/Promega
Average 90 stars, based on 1 article reviews
pgem 4 - by Bioz Stars, 2026-05
90/100 stars
  Buy from Supplier
32 p]dctp  (DuPont de Nemours)
90
DuPont de Nemours 32 p]dctp
Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell <t>genomic</t> <t>DNA</t> hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.
32 P]Dctp, supplied by DuPont de Nemours, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/32 p]dctp/product/DuPont de Nemours
Average 90 stars, based on 1 article reviews
32 p]dctp - by Bioz Stars, 2026-05
90/100 stars
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Image Search Results


Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell genomic DNA hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.

Journal:

Article Title: Insulin receptor substrate 2 plays a crucial role in ? cells and the hypothalamus

doi: 10.1172/JCI200421484

Figure Lengend Snippet: Generation of βHT-IRS2 mice. (A) Schematic representation of the 3 steps in our gene-targeting strategy. (B) Southern blot analysis. Left: SacI-digested ES cell genomic DNA hybridized with probe A. The 4-kb band corresponds to the WT (+) allele, and the 3.2-kb band to the first step of the mutant (neo) allele. Middle: SpeI- and EcoRV-digested ES cell genomic DNA hybridized with probe B. The 11.8-kb band corresponds to the WT (+) allele, the 2.9-kb band to the (neo) allele, and the 1.6-kb band to the second step of the mutant (Δ) allele. Right: HindIII-digested ES cell genomic DNA hybridized with probe C. The 17.3-kb band corresponds to the WT (+) allele, the 15.2-kb band to the mutant (Δ) allele, and the 6-kb band to final step of the mutant (neoΔ) allele. (C) PCR analysis of genomic DNA to detect Cre-mediated recombination. K, kidney; L, liver; M, muscle; A, adipose tissue; S, spleen; HT, hypothalamus; H, heart; Lu, lung; I, islet. (D) RT-PCR of Cre and Irs2 expression in liver, skeletal muscle, adipose tissue, hypothalami, and pancreatic islets of WT, RIP-Cre, IRS2lox/lox, and IRS2lox/lox/RIP-Cre (βHT-IRS2) mice. (E) Western blot analysis of Irs2 in pancreatic islets (upper panel) and hypothalami (lower panel) from control and βHT-IRS2 mice.

Article Snippet: To construct the targeting vectors for the Irs2 gene, a 129/Sv mouse genomic DNA library packaged in Lambda DASH II (BD Biosciences — Clontech) was screened with 5′-side and 3′-side mouse genomic DNA as probes (probes C and B, respectively, in Figure A).

Techniques: Southern Blot, Mutagenesis, Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot